منابع مشابه
Immunofluorescence techniques.
anTiBOdies An antibody is a protein complex produced by B cells that initiates an immune response against a target antigen. The basic organization of an antibody includes two functional domains that, together, resemble the letter Y (Figure 1, left). The Fab (fragment having the antigen binding site) domain makes up the arms of the Y, and at the end of each arm is a variable region responsible f...
متن کاملTechniques of immunofluorescence and their significance.
1) Skin or mucosal biopsy specimens are usually obtained by 3-5 mm punch or surgical biopsy. 2) Biopsy specimens are snap frozen. If there is a delay between biopsy and snap freezing, the specimen should be kept in cold saline. 3) Frozen 4-6μ sections are cut on a cryostat and placed on a glass slide before being air-dried for 15 minutes. 4) After rinsing in phosphate buffered saline (PBS), pH ...
متن کاملImmunofluorescence Techniques as an Aid to the Diagnosis of Gonorrhoea.
The technique of staining antigens with a fluorescent dye coupled to specific antibody was first applied to the identification of N. gonorrhoeae by Deacon, Peacock, Freeman, and Harris (1959). From this and further studies they concluded that the fluorescent antibody (F.A.) technique was superior to conventional cultural diagnostic methods and afforded a considerable saving in labour and materi...
متن کاملIntracellular distribution of Tankyrases as detected by multicolor immunofluorescence techniques
Poly(ADP-ribose) polymerases are a family of enzymes that catalyze the conversion of NAD+ into ADP-ribose. Among them, Tankyrases have been found to bind to centrosome, mitotic spindle and microsome proteins, in the cytoplasm, and to telomeres in the nucleus, where they play a relevant role in telomere metabolism. However, their precise intracellular localization during interphase has not been ...
متن کاملComparison of techniques and immunoreagents used for indirect immunofluorescence and immunoperoxidase identification of enteroviruses.
Peroxidase-conjugated antibodies were found to be as sensitive as those conjugated to fluorescein-isothiocyanate (FITC) for identification of selected enterovirus types by the indirect technique. Peroxidase conjugates, however, were found to give fewer nonspecific reactions. The reasons for the higher specificity of the immunoperoxidase technique appear to be related to the relative size, charg...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Journal of Investigative Dermatology
سال: 2013
ISSN: 0022-202X
DOI: 10.1038/jid.2012.455